Sixteen strains of large fungi collected in the field from Huaihua were isolated and purified . Congo red staining , the hydrolysis circle and colony diameter ratio (H/C value ) were analysed on CMC solid medium to screen cellulase producing fungal strains . By determining the filter paper enzyme activity (FPA ) in its supernatant of liquid fermentation culture , higher cellulase activity strain 01 were obtained ultimately . Factors including culture temperature , pH and incubating time affecting the production of cellulase had been studied by using the rice and CMC as carbon source . The results showed that the best culture temperature was 27℃ , the optimal pH of culture medium and incubating time was 5.0 and 6d , respectively . Under the optimal condition , the FPA reached 580.0IU/mL . In this article , it showed that fungal 01 could been a candidate for future studying cellulase and feed production .%为了获得高产纤维素酶菌株，有效地开发和利用纤维素资源.本研究通过对野外采集的大型真菌进行分离纯化，获得了16个菌株.利用CMC固体培养、刚果红染色，测量水解圈与菌落直径的比值（H/C值），对获得的菌株进行初筛；通过液体发酵培养，测定其上清液中的滤纸酶活力（FPA ），对菌株进行复筛，最终获得了纤维素酶活性较高的菌株01.以稻草和羧甲基纤维素为碳源，研究了培养温度、pH值、培养时间对真菌菌株01产纤维素酶的影响.结果表明，该菌株产纤维素酶的最适培养温度为27℃， pH值为5.0，培养时间为6 d ，菌株01的滤纸酶活性达到580.0 IU/mL.因此，真菌01可作为纤维素酶研究和饲料加工等生产的备选菌株.
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