In order to know the key functional regions in the aphid transmission (AT) protein with which aphids transmit barley yellow dwarf virus (BYDV), BYDV AT protein was analyzed with many sequence analysis tools. The results showed that there were two highly conservative sequences "VDSS" and "KRFFEY" at the read through protein in AT protein of GAV,MAV and PAV strains,the sequence from "VDSS" to "KRFFEY" varied among different strains, and the O-glycosylation and phosphorylation sites of AT protein were mainly distributed in this region, so it was deduced that the sequence from "VDSS" to "KRFFEY" might be the functional region deciding the difference of aphid transmission characteristics between strains. The AT protein was mainly located at cell nucleus. The coiled-coils were found in the AT proteins of three strains, and the sequences "EYEAA" and the location of the coiled-coils near the 510 aa were highly conservative. Thus, the coiled-coil structure of AT protein could play an important role in the process of gene expression regulation and molecular recognition during the aphid transmission of BYDV.%为探讨大麦黄矮病毒(BYDV)蚜传(AT)蛋白参与介导蚜虫传播BYDV过程的关键功能区,利用Clustal等多种序列分析工具对BYDV AT蛋白进行综合分析.结果显示,在GAV株系、MAV株系和PAV株系的AT蛋白通读蛋白区均具有高度保守序列“VDSS”和“KRFFEY”,两保守区之间的氨基酸序列在株系间存在特异性变异,AT蛋白的主要糖基化和磷酸化位点亦分布在该区,表明该区域可能是决定株系间蚜传特性差异的功能区.另外分析表明,AT蛋白具有卷曲螺旋结构,在动物细胞中主要定位于细胞核内,其第510位氨基酸残基附近的卷曲螺旋结构不但在序列上高度保守(EYEAA)而且位置上也高度相近,说明该结构可能在AT蛋白介导蚜虫传播过程中起调控基因表达或分子识别的作用.
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