黔产9种薯蓣属植物分子鉴别及亲缘关系研究

摘要

In order to reveal the genetic relationship between 9 species of Dioscorea in Guizhou province,the molecular identification of the species was conducted using the sequence fragments of chloroplast genes matK,rbcL and psbA-trnH.The results showed that the lengths of matK,psbA-trnH and rbcL in 9 species of Dioscorea in Guizhou province were 1 076-1 144 bp,361-382 bp and 1 160-1 209 bp.When the vacancy was treated as deletion,the ratio of the mutation site in the total length was 14.3％,7.8％ and 8.7％,respectively.Phylogenetic analysis showed that 9 species of Dioscorea derived from the 31 provenances were divided into four groups.Among them,Dioscorea bulbifera L.belonged to the cluster of Sect.Opsophyton Uline.Dioscorea melanophyma Prain et Burkill and Dioscorea henryi (Prain et Burkill) C.T.Ting belonged to the cluster of Sect.Lasiophyton Uline.Dioscorea subcalva Prain et Burkill and Dioscorea nitens Prain et Burkill belonged to the cluster of Sect.Shannicorea Prainet Burkill.Dioscorea glabra Roxb.,Dioscorea cirrhosa Lour.,Dioscorea japonica Thunb.and Dioscorea opposita Thunb.belonged to the cluster of Sect.Enantiophyllum Uline.The results show that matK,rbcL and psbA-trnH have obvious guidance for systematic classification of Dioscorea.%为揭示黔产薯蓣属植物9个种之间的亲缘关系,根据叶绿体matK、rbcL、psbA-trnH序列片段对其进行种间分子鉴别研究.结果表明,黔产薯蓣属植物9个种的matK、psbA-trnH、rbcL序列长度分别为1 076～1 144 bp、361 ～382 bp、1 160～1 209bp,当空位始终做缺失处理时,其变异位点分别占序列总长度的14.3％、7.8％及8.7％.系统进化分析显示,来自9个种31份样本的薯蓣属植物分为了4个大组.其中,黄独单独为一组,为基生翅组;黑珠芽薯蓣和高山薯蓣聚为一组,为复叶组;光亮薯蓣和毛胶薯蓣聚为一组,为顶生翅组;光叶薯蓣、薯莨、日本薯蓣以及薯蓣聚为一组,为周生翅组.表明matK、rbcL、psbA-trnH序列片段对薯蓣属植物的系统分类具有明显的指导价值.