单味中药对兔球虫卵囊孢子化抑制效果的研究

摘要

To investigate the inhibition effect of the different concentration of single Chinese herbal medicine on the sporulating of rabbit coccidia oocysts at different times,a series of experiments were performed. Firstly,Under the same concentration culture conditions,the sporulated rate of rabbit coccidia oocysts gradually went up with time in all choosed Chinese herbal medicin,except for fructus bruceae. In other words,suppression effect of fructus bruceae was significant,and herba agrimoniae,radix dichroae,herba artemisiae annuae,herba verbenae,fructus quisqualis,radix bupleuri had a certain suppression effect. Then Chinese herbal medicine was washed and cultured for 3 days,and the sporulated rate of rabbit coccidia oocysts had been on the rise in all groups,which showed that some Chinese herbs had some certain suppression effect on the sporulating of rabbit coccidia oocysts,but they did not effectively eliminate the oocysts. Additionally,inhibition test was performed under different concentration culture conditions.The results showed that the sporulated rate of oocysts were changed significantly with increase in the concentration by herba agrimoniae, radix dichroae,herba artemisiae annuae and fructus bruceae,especially the suppression effect of 100% concentration was better than 50% and 25%. By contrast,the sporulated rate of oocysts increased were not significantly with the above concentration by herba verbenae,fructus quisqualis and radix bupleuri.%研究单味中药在不同时间及不同浓度下对兔球虫卵囊孢子化抑制效果。首先，在同浓度单味中药培养的条件下进行观察，发现随着时间的延长，除鸦胆子中卵囊孢子化率没有升高外，其余中药球虫卵囊孢子化率均有不同程度的升高；鸦胆子对兔球虫卵囊孢子化有显著的抑制作用，仙鹤草、常山、青蒿、马鞭草、使君子和柴胡有一定的抑制作用；各组洗去中药继续培养3 d，发现各组卵囊孢子化率均呈上升趋势。这表明，虽然有些中药对兔球虫孢子化有一定的抑制作用，却不能杀灭卵囊。不同浓度中药抑制实验结果表明，仙鹤草、常山、青蒿、鸦胆子随浓度增高孢子化率改变明显，100%浓度抑制球虫卵囊孢子化的效果优于50%和25%浓度，而马鞭草、使君子、柴胡随浓度变化改变不明显。