大庆龙凤湿地底泥氨氧化细菌与氨氧化古菌多样性分析

摘要

To study the diversity of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) in sediment of Longfeng wetland in Daqing,amoA clone libraries of AOB and AOA were constructed. The libraries were analyzed by PCR-based restriction fragment length polymer-phism(RFLP)technique. The results showed that 3 OTUs of AOB and AOA were obtainted from Longfeng wetland sediment,respectively. All sequences of AOB belonged to Nitrosomonas,and all sequences of AOA belonged to Crenarchaeota,and it had the high sequence homology with the sequences that were obtained from estuary sediments.%为研究湿地底泥中氨氧化细菌（Ammonia-Oxidizing Bacteria，AOB）与氨氧化古菌（Ammonia-Oxidizing Archaea，AOA）的群落多样性，构建AOB与AOA的amoA 基因克隆文库，同时利用限制性片段长度多态性（RFLP）技术将克隆文库阳性克隆子进行归类并测序。结果发现，从湿地底泥中均得到3个AOB与AOA的可操作分类单元（OTU）。其中，所有的AOB均属于亚硝化单胞菌属（Nitrosomonas），而AOA属于泉古菌门（Crenarcharota），与河口沉积物等环境中获得的序列具有较高同源性。