在适当的条件下,强力霉素可与镁离子形成具有强荧光的络合物,在溴化十六烷基三甲铵胶束体系中,荧光可进一步增强.据此建立了一种测定强力霉素残留的新方法.在优化条件下,强力霉素线性范围为0.2～10 mg/L,相对标准偏差为0.9%～3.9%,检出限为12μg/kg,加标回收率90.5%～107.3%.该方法已应用于猪肉强力霉素残留的测定.%Doxycycline can form hyperfluorescence compound with Mg2+ under proper conditions. The fluorescence of the compound can be futher increased in cetyltrimethyl-ammonium bromide micellar. A new assay was developed to determine the doxycycline residue based on this principle. Under the optimum conditions, the linear dynamic range was 0. 2-10 mg/L; the relative standard deviation was between 0. 9％ and 3.9％; the detection limit was 12 μg/kg; the recoveries of spiked sample were between 90.5％ and 107.3％. The method has been applied to the determination of doxycycline residue in pork.
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