复发性生殖器疱疹患者血清白细胞介素2水平及外周血T淋巴细胞亚群变化规律研究

摘要

Objective To detect the expression of interleukin-2( IL-2 ) and T lymphocyte subsets in peripheral blood of patients with recurrent genital herpes ( RGH ), and to discuss the immunological mechanism of repeated recurrent genital herpes. Methods One hundred cases of RGH patients were selected as research objects. IL-2 levels were detected by enzyme linked immunosorbent assay ( ELISA ), and the T lymphocyte subsets were detected by flow cytometry. Another 100 healthy volunteers without infectious diseases served in control group. Results The serum IL-2 level of RGH group was significantly lower than that of normal control group ( P < 0. 01 ); Peripheral serum CD3 + , CD4+ ,CD4+/CD8 + T expression in RGH group were significantly lower than those of the control group ( P < 0. 05 ); while the CD8 + T cell expression was significantly higher than that of the control group( P < 0.05 ); peripheral blood CD3 + , CD4+ , CD4+/CD8+ T cells of elderly patients in RGH group was significantly lower than that of young patients( P < 0. 05 ), and CD8 + T cells was significantly higher than that of young patients ( P < 0. 05 ). Conclusion The IL-2 and T lymphocyte subsets of patients with RGH were abnormal. There are cellular immune dysfunction and immune suppression in the patients with RGH.%目的 观察复发性生殖器疱疹(recurrent genital herpes,RGH)患者血清白细胞介素2(interleukin-2,IL-2)水平及外周血T淋巴细胞亚群的变化规律,探讨RGH患者反复发作的免疫学机制.方法 选择100例RGH患者,采用酶联免疫吸附测定方法检测IL-2,采用流式细胞仪检测T淋巴细胞亚群.对照组100例来自无感染性疾病的健康志愿者.结果 RGH组血清IL-2水平明显低于正常对照组(P＜0.01).RGH组外周血清中的CD3+、CD4+、CD4+/CD8+T细胞表达较对照组明显降低(P＜0.05);而CD8+T细胞表达则明显高于对照组,差异有统计学意义(P＜0.05).RGH组老年患者外周血CD3+、CD4+、CD4+/CD8+T细胞明显低于青年患者(P＜0.05),而CD8+T细胞明显高于青年患者(P＜0.05).结论 RGH患者血清中IL-2水平及外周血T淋巴细胞亚群水平异常,患者体内存在细胞免疫功能失调及免疫抑制现象.