建立理冲生髓饮中多糖及水溶性蛋白质质量分数测定的方法．测定理冲生髓饮多糖对葡萄糖的换算因子，利用苯酚－硫酸法测定多糖的质量分数；采用考马斯亮蓝G－250染色法测定理冲生髓饮中可溶性蛋白质的质量分数．多糖标准曲线方程为A ＝12．839 C－0．0085（r ＝0．9993），蛋白质标准曲线方程为A ＝1．271 C－0．0331（r ＝0．9992），两者分别在0．01～0．1、0．1～1．0 g／L有良好的线性关系．测得理冲生髓饮中多糖质量分数为8．53％，水溶性蛋白质质量分数为0．35％．该两种方法操作简便、灵敏、快速、准确，重复性好，可用于理冲生髓饮中多糖、水溶性蛋白质的质量分数测定．%To establish a method for the determination of polysaccharide and water -soluble protein in Lichong Shengsui Drink ( LCSSD) , the conversion factor of LCSSD polysaccharide to glucose was obtained with refined polysaccharide , and the content of LCSSD polysaccha-ride was determined by using phenol -sulfuric method .The content of protein was deter-mined by using the dying method with Coomassie brilliant blue G -250.The polysaccharide standard curve equation was A =12.839 C -0.008 5 (r =0.999 3) and protein standard curve equation was A =1.271 0 C -0.033 1 (r =0.999 2).Respectively the linear range was 0.01~0.1 g/L and 0.1~1.0 g/L.The content of polysaccharide was 8.53%, and the content of protein was 0.35%.The method is simple, fast, accurate and reproduci-ble, and it should be helpful for the determination of polysaccharide and water -soluble pro-teins in LCSSD .
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