不同预处理方法对剩余污泥厌氧发酵产氢的影响

摘要

The excess activated sludge was pretreated by six-methods（acid,alkaline,heat,aeration,chloroform and 2-bromoethanesulfonate（BES）,respectively） in batch tests to evaluate and compare their suitability in the enrichment of hydrogen-producing bacteria.The experimental results showed that,at 35 ℃ and initial pH 7.0,the hydrogen yields of the pretreated sludge（except BES） were higher in comparison with the control test during the fermentative hydrogen production.In all tests,for fermentative hydrogen production from glucose,the sludge pretreated by acid and alkaline could obtain the maximal hydrogen production potential,using glucose as the substrate,and the hydrogen yield from glucose and specific hydrogen yield in terms of mixed liquor volatile suspend solid（MLVSS） reached 1.51 mol/mol and 27.29 mol/gMLVSS,respectively.It was concluded that the pretreatment methods led to different fermentation types.Butyric-acid type fermentation was achieved by acid and alkaline pretreatments,an ethanol-type fermentation was observed using aeration,BES pretreatments and the control,while mixed-acid type fermentation was achieved by heat and CHCl3pretreatments.%对种泥进行预处理,能去除不产芽孢的耗氢菌,可以达到加快有机废水发酵生物制氢系统启动进程,提高污泥产氢效能的目的.为寻求适宜的种泥预处理方法,利用摇瓶发酵实验,考察城市污水处理厂好氧活性污泥分别经酸、碱、热、曝气、CHCl3和二溴乙烷磺酸钠（BES）预处理后,其利用葡萄糖发酵产氢的特性.结果表明,在初始pH 7.0、葡萄糖质量浓度10 g/L、MLVSS接种量2 g/L条件下,35℃培养42 h,经酸预处理的种泥表现出最好的发酵产氢性能,其葡萄糖的氢气转化率达1.51 mol/mol,污泥的比产氢率为27.