类风湿关节炎患者血清TNF-α、IL-2及IL-13变化的价值研究

摘要

目的:探讨类风湿关节炎患者血清肿瘤坏死因子-α(TNF-α)、白介素(IL)-2及IL-13变化,为临床诊断及治疗提供参考.方法:选自我院收治的类风湿关节炎患者60例作为观察组,另选自我院同期健康体检者60例作为对照组.采用酶联免疫吸附试验双抗体夹心法检测观察组患者治疗前后及对照组血清中TNF-α、IL-2及IL-13变化,并与类风湿关节炎疾病活动度指标血浆红细胞沉降率(ESR)、C反应蛋白(CRP)水平及DAS28评分进行相关性分析.结果:治疗前观察组血清TNF-α、IL-2及IL-13水平均明显高于对照组(P＜0.05),治疗后观察组血清TNF-α、IL-2及IL-13水平均较治疗前明显降低(P＜0.05),且与对照组相比差异无统计学意义(P＞0.05).治疗前,类风湿关节炎患者发病期血清TNF-α、IL-2及IL-13变化与血浆ESR、CRP水平及DAS28评分呈正相关(P＜0.05).结论:类风湿关节炎患者血清TNF-α、IL-2及IL-13均明显升高,且与类风湿关节炎疾病活动度密切相关,患者经治疗后可得到有效改善,值得临床借鉴.%Objective:To explore the changes of serum TNF-α,IL 2 and IL-13 in patients with rheumatoid arthritis (RA),and to provide reference for clinical diagnosis and treatment.Methods:A total of 60 patients with rheumatoid arthritis admitted to our hospital from December 2016 to December 2017 were selected as the observation group,and another 60 healthy people in the same period were selected as the control group.Enzyme-linked immunosorbent assay (ELISA) double antibody sandwich method was used to detect the changes of serum TNF-α,IL-2 and IL-13 in the observation group before and after treatment,and was correlated with rheumatoid arthritis disease activity index,plasma ESR,CRP level and DAS28 score.Results:Before treatment,serum TNF-α,IL-2 and IL-13 were significantly higher than those in the control group (P＜0.05),the observation group of serum TNF-α,IL-2 and IL-13 levels were significantly lower than those before treatment (P＜0.05),and compared with the control group,the difference was not statistically significant (P＞ 0.05).Before treatment,the changes of serum TNF-α,IL-2 and IL-13 during the onset of rheumatoid arthritis were positively correlated with the level of plasma ESR,CRP and DAS28 (P＜0.05).Conclusion.:the serum levels of TNF-α,IL-2 and IL-13 in rheumatoid arthritis patients are significantly increased,and are closely related to the activity of rheumatoid arthritis.Patients can be effectively improved after treatment,which is worthy of clinical reference.