活血祛痰法对自发性高血压大鼠心肌 线粒体膜的影响

摘要

【Objective】To explore the effects of blood-circulation pro motingand phlegm removing treatment on the structure, activity of Ca2+-Mg2+ATPase and malondialdehyde(MDA) content of mitochondria membrane in spontaneo us hypertensive rats (SHR).【Methods】Spontaneous hypertensive rats (SHR) were c las sified into 14-week-old model group(Group C),28-week-old model group(Group D) an d treatment group (treated with blood-circulation promoting and phlegm removing Chinese herbal medi cine,Group E).Normal Wistar rats 14 weeks old (Group A)and 28 weeks old(Group B) served as the controls .The structure of myocardial mitochondria membrane were analyzed quantitatively by transmission elec tron microscope,and the activity of Ca2+-Mg2+ATPase and MDA content were also detected.【Results】Specific surface area of mitochondria was smaller, the activity of Ca2+-Mg2+ATPase was lower and MDA content was higher in SHR than the controls.MDA content increased as the duration of disease prolonged. Chinese herbal medicine could counteract the above changes.【Conclusion】Blood-c irculation promoting and phlegm removing herbs can partially c ounteract the progress of left ventricular hypertrophy in SHR.%【目的】探讨活血祛痰法对自发性高血压大鼠(SHR)心肌线粒体超微结构及其Ca2+-Mg2+ATP酶、膜脂质过氧化的作用。【方法】以正常Wistar大鼠为对照组，将SHR分为14周龄组、28周龄组和中药治疗组，分别在14周和28周取材，透射电镜分析线粒体膜形态计量，并测定线粒体膜Ca2+-Mg2+ATP酶活性、膜丙二醛含量。【结果】SHR各组线粒体比表面小于对照组，心肌线粒体Ca2+-Mg2+ATP酶活性均低于正常对照组，而丙二醛含量高于正常对照组，且随着SHR病程延长而呈上升趋势；中药治疗能降低该趋势。【结论】活血祛痰法能阻滞左室肥厚的病理进程。