淫羊藿在骨膜附加活性材料nHAC／PLA修复颌骨缺损中的影响

摘要

【目的】观察淫羊藿对纳米羟基磷灰石/胶原/聚乳酸（nHAC/PLA）在骨缺损修复过程中的成骨作用。【方法】制备16只兔双侧下颌骨10mm×8mm贯穿性缺损模型，随机分为中药淫羊藿组与对照组，2组左侧缺损不添加材料，右侧缺损处添加活性材料nHAC/PLA。术后2、4、8、12周分别处死动物，从大体、影像学、组织学方面进行观察，并计量新骨面积。【结果】影像学观察结果显示淫羊藿右侧组、对照右侧组、淫羊藿左侧组2、4、8、12周缺损区密度均高于对照左侧组。组织学观察可见淫羊藿右侧组、对照右侧组各时间点骨缺损区新骨形成均优于左侧组，新生骨及新生血管的量多。随着时间延长，淫羊藿右侧组、对照右侧组有大量骨样组织长入活性材料，骨成熟度增高，淫羊藿右侧组可见残留材料逐渐被活跃生长的骨组织包围并替代。分别与同侧对照组比较，淫羊藿左、右侧组新骨面积均显著升高，差异有统计学意义（P＜0．05）；同组左侧与右侧分别比较，差异均有统计学意义（P＜0．05）。【结论】淫羊藿在骨缺损修复期间有良好的促进骨愈合的作用， nHAC/PLA活性材料具有良好的生物相容性、骨传导性及骨诱导活性，有望应用于临床修复骨缺损。%Objective To investigate the influence of Herba Epimedium, a kidney-strengthening Chinese herb, on the regenerate ossification of nano-hydroxyapatite/collagen/ polylactic acid (nHAC/PLA) in the repair of rabbit mandibular defect. Methods Sixteen rabbit models with 10 mm × 8 mm subperiosteal mandibular defect were established. The rabbits were randomly divided into 2 groups, namely Herba Epimedium group and control group. In both groups, the left mandibular defect region (short for left side) was not filled with any material, while the right mandibular defect region ( short for right side) was filled with nHAC/PLA. On week 2, 4, 8, 12 after modeling, rabbits in each group were killed, and then the macro-view, imageology and histology of the bilateral mandibles were observed. The data of the regenerate bone areas were calculated and analyzed with analysis of variance (ANOVA) . Results The imageological results showed bone mineral density in the right side of Herba Epimedium group and the control group, and in the left side of Herba Epimedium group were higher than those in the left side of the control group on week 2, 4, 8, 12 after modeling. The histological results showed that the regenerate ossification in the right side of Herba Epimedium group and the control group was superior to that in the left side of the corresponding groups, with more regenerative bone and vessels. In the right side of Herba Epimedium group and the control group, amount of bone-like tissues were grown into nHAC/PLA, and the maturity degree of bone tissue was increased with the prolongation of time. And the changes were more obvious in the right side of Herba Epimedium group, showing as the remaining of nHAC/PLA having been surrounded and replaced by regenerative bone tissue. Regenerative bone area was increased in both sides of Herba Epimedium group, which differed from the same side of the control group (P<0.05) . The differences of the regenerative bone area were also significant between the left side and right side of the same group (P<0.05) . Conclusion Herba Epimedium can effectively accelerate the repair of mandibular defect, and nHAC/PLA is of good biocompatibility, osteoinduction and bone conduction, and can be expected for bone defect repairing in clinic.