通络糖泰方对糖尿病周围神经病变大鼠炎性细胞因子的影响

摘要

Objective To investigate the effect of Tongluo Tangtai Recipe(TTR)on inflammatory cytokines in rats with diabetic peripheral neuropathy(DPN). Methods Rat model of spontaneous DPN was established by feeding high fat diet. The rats were divided into 5 groups , namely model group , western medicine(metformin 100 mg·kg-1·d-1+ methycobal 0.2 mg·kg-1·d-1) group, and low-, middle- and high-dose TTR groups (TTR in the dosage of 0.72, 1.44, 2.88 g·kg-1·d-1 respectively). Rats of the normal group and model group were treated with normal saline orally, and the medication groups were given the corresponding medicine according to the experimental design. After intragastric administration for 4 weeks, sciatic motor/sensory nerve conduction velocity was detected with electrophysiological examination method, and tumor necrosis factor alpha(TNF-α), interleukin-6(IL-6), C-reactive protein(CRP), myelin basic protein(MBP) levels in the serum were examined by enzyme-linked immunosorbent assay(ELISA). Results TTR in various dosages could reduce the serum levels of MBP and inflammatory cytokines of TNF-α, IL-6 and CRP, and increase sciatic nerve conduction velocity (P<0.05 or P<0.01 compared with those in the model group), the high dose showing the strongest effect. Conclusion TTR could inhibit inflammation reaction and nerve demyelination, and improve nerve structure and conduction function, which may contribute to the preventive and therapeutic mechanism of TTR for DPN.%【目的】探讨通络糖泰方对糖尿病周围神经病变（DPN）大鼠炎性细胞因子的影响。【方法】选用自发性2型糖尿病GK大鼠，建立由高脂饲料喂养的 DPN 大鼠模型，随机分为5组：模型组，通络糖泰方高、中、低剂量组（剂量分别为2.88、1.44、0.72 g·kg-1·d-1），西药组（100 mg·kg-1d·-1二甲双胍+0.2 mg·kg-1·d-1弥可保）。并选用Wistar大鼠设为正常组。分别给予相应处理4周后采用神经电生理检测大鼠坐骨神经运动/感觉神经传导速度；采用酶联免疫吸附试验检测血清肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、 C反应蛋白（CRP）、髓鞘碱性蛋白（MBP）水平。【结果】通络糖泰各剂量组可降低血清炎性细胞因子水平，降低血清MBP水平，提高坐骨神经传导速度，其中高剂量组作用最显著。【结论】通络糖泰方可减轻炎症反应，减少神经脱髓鞘病变，改善神经结构和传导功能，从而达到防治DPN发生发展的作用。