围绝经期非器质性失眠妇女肝郁分级与雌激素r受体α、β,GPR30的相关性研究

摘要

[目的]探讨围绝经期非器质性失眠肝郁分级与雌激素受体α、β(ERα、ERβ),G蛋白耦联受体30(GPR30)mRNA及蛋白表达的相关性.[方法]收集127例围绝经期非器质性失眠妇女的四诊信息及匹兹堡睡眠质量指数(PSQI)评分,参照《证素辨证学》进行肝郁证素积分和肝郁分级,运用实时荧光定量(RT-qPCR)法和免疫印迹(WB)法检测外周血单个核细胞上的GPR30、ERα、ERβmRNA及蛋白的表达水平.[结果](1)ERαmRNA、ERβmRNA、GPR30 mRNA相对表达量在不同肝郁分级间差异均无统计学意义(P>0.05);ERαmRNA/ERβmRNA比值随肝郁分级增高而降低,其在不同肝郁分级间差异有统计学意义(F=6.710,P<0.001).(2)ERα、ERβ、GPR30蛋白相对灰度值在不同肝郁分级间的差异均无统计学意义(P>0.05);ERα/ERβ蛋白相对灰度值比值随肝郁分级增高而降低,其在不同肝郁分级间差异有统计学意义(P<0.05).[结论]围绝经期非器质性失眠妇女肝郁的病理基础可能与ERα/ERβ比值下降相关.%Objective To explore the correlation of liver depression grading with estrogen receptor alpha and beta(ERα,ERβ)and G protein-couple receptor 30(GPR30)of the perimenopausal women suffering from non-organic insomnia. Methods A total of 127 perimenopausal women suffering from non-organic insomnia were enrolled into the study. The data collected by four diagnostic methods and the scores of Pittsburgh Sleep Quality Index(PSQI)were used for the scoring and grading of liver depression according to Syndrome Element Differentiation. The mRNA and protein expression levels of ERα,ERβand GPR30 in the mononuclear cells of peripheral blood from the patients were detected with real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and Western blot (WB)method respectively. Results (1)The differences of mRNA levels of ERα,ERβ and GPR30 were insignificant among the patients with different degrees of liver depression (P > 0.05). ERα mRNA/ERβ mRNA ratio was decreased with the increase of liver depression grading,the differences being significant (F = 6.710, P < 0.001). (2) The differences of protein levels of ERα, ERβ and GPR30 were insignificant among the patients with different degrees of liver depression (P > 0.05);ERα/ERβ protein gray value ratio was decreased with the increase of liver depression grading, and the differences were significant (P < 0.05) . Conclusion The decline of ERα/ERβ is probably contributed to the pathological basis of liver depression in perimenopausal non-organic insomnia women.