三阴性乳腺癌组织中干细胞CD44+/CD24-/low表型与预后的关系

摘要

Objective To investigate the expressions of CD44+/CD24-/low phenotype in human triple-negative breast cancer and the relationship of CD44+/CD24-/low with clinic pathological features and prognosis in the triple-negative breast cancer.Methods 53 paraffin specimens of triple negative breast cancer patients were collected from January 2008 to December 2009. 53 paraffin specimens from non-triple negative breast cancer patients of same period corresponding clinical staging were randomly selected.The expression of CD44+/CD24-/low phenotype between the triple negative breast cancer patients and non-triple negative breast cancer patients by immunohistochemical technique for CD44 and CD24 staining were statistically analyzed so as to explore the relationship of CD44+/CD24-/low with clinic pathological features and prognosis in the triple-negative breast cancer.Results The positive rate of CD44+/CD24-/low in the triple-negative breast cancer of 54.7%.Discoveried that the proportion of CD44+/CD24-/low cells in the triple negative breast cancer patients group was significantly higher than those of in the non-triple negative breast cancer patients. The patients with gradeⅢ were 15 patients (28.3% ) in the TNBC group,and were 5 patients (9.4% ) in the NTNBC group (P<0.05) .In TNBC group, significant differences(P<0.05) were observed in recurrence 24.5% , systemic organ metastases 17% in the CD44+/CD24-/low (+) higher than in the CD44+/CD24-/low (-).In TNBC group, significant differences(P<0.05) were observed in the 5-year OS 62% , DFS 51% in the CD44+/CD24-/low (+) lower than in the CD44+/CD24-/low(-).Conclusion The recurrence , distant metastasis and poor prognosis of triple-negative breast cancer was associated with the elevated expression of CD44+/CD24-/low.%目的：探讨CD44+/CD24-/low表型在三阴性乳腺癌（triple-negative breast cancer，TNBC）中的表达情况及其与三阴性乳腺癌的临床病理特征及预后的关系。方法从青岛市中心医疗集团乳腺癌数据库中筛选出2008年1月至2009年12月53例三阴性乳腺癌病人的石蜡标本，同时随机选取53例同时期相应临床分期的非三阴性乳腺癌（NTNBC）病人的石蜡标本。应用免疫组化技术进行CD44、CD24染色，将CD44+/CD24-/low表型在两组病人中的表达情况进行统计学分析，探讨其与三阴性乳腺癌的临床病理特征及预后的关系。结果 CD44+/CD24-/low（+）在TNBC组中为54.71%，明显高于NTNBC组(45.28)（P<0.05）。在TNBC组，临床病理分期Ⅲ期在 CD44+/CD24-/low（+）中有15例（28.30%），高于CD44+/CD24-/low（－）（9.43%）（P<0.05）；局部复发率在CD44+/CD24-/low（+）和CD44+/CD24-/low（－）中分别为24.52%和5.66%（P<0.05）；远处转移率分别为16.98%和3.77%（P<0.05）。TNBC组中，CD44+/CD24-/low（+）5年OS（62.00%）、DFS（51.00%）均低于CD44+/CD24-/low（－）5年OS（80.00%）、DFS（75.00%），两组间的差异有统计学意义。结论 CD44+/CD24-/low的高表达可能与三阴性乳腺癌的复发、转移及预后差有相关性。