自然感染巴氏杆菌藏羊组织器官的病理学研究

摘要

为了对甘肃省武威市某羊场藏羊自然爆发的疑似巴氏杆菌病的病羊进行确诊和病理学研究，试验采用染色镜检、分离培养、生化试验鉴定、动物试验、病理组织制作和染色等方法，对采集的病料分别进行了细菌学检测和病理组织学观察。结果发现：瑞氏染色可见两端着色椭圆形短杆菌，分离培养、生化试验鉴定、动物试验均表明其特性为多杀性巴氏杆菌。病理组织学变化为：肺脏呈纤维素性-化脓性肺炎，心脏呈程度不等的变质性心肌炎和化脓性心肌炎，肝脏呈程度不等变质性肝炎，脾脏呈程度不等坏死，肾脏呈急性肾小球肾炎和局灶性化脓性肾炎，全身淋巴结呈浆液-出血性淋巴结炎。同时，病羊重要实质器官肝、脾、肾、淋巴结等组织内均出现大量单核巨噬细胞的广泛增生。病变组织中间质胶原纤维肿胀、断裂、崩解，血管壁纤维素样坏死。结果表明，本次武威市某羊场藏羊自然爆发疾病为羊巴氏杆菌病，其主要病理组织学变化特点为肺脏呈纤维素性-化脓性肺炎和心脏的变质性-化脓性心肌炎。%The tissue samples from pasteurellosis suspected Tibet sheep were tested by pathological method in Wuwei City,Gansu Province.The result of bacterium analysis showed that the disease pathogen of the Tibetan sheep was Pasteurellamultocida.The diseases of the Tibetan sheep were diagnosed as pas-teurellosis.The pathological changes indicate following characteristics:the lungs emerge fibrinous pneu-monia and suppurative pneumonia mixture performance.The hearts have varying degrees myocarditis.The livers emerge varying degrees hepatitis.The spleens appear acute splenitis.The kidneys have viously degen-eration and acute glomerulonephritis and suppurative nephritis.The lymph nodes emerge serious acute lym-phadenitis.At the same time,there are large number of macrophages and multiple nuclear giant cells to in-tersperse in livers,kidneys,spleens and lymph nodes.The blood vessels of important tissues occurred fi-brinoid necrosis.The collagen fibers and elastic fibers are swelled and collapsed in this tissues.