微弧氧化和碱处理对钛的细胞粘附和骨植入行为的影响研究

摘要

考察了微弧氧化处理和碱处理对钛表面形貌、涂层成分、羟基磷灰石沉积能力、细胞黏附、骨内植入的影响。结果表明，微弧氧化和碱复合处理后的钛表面 TiO2薄膜呈现大量裂缝，表现出表面含大量OH-极性基团的纳米多孔网状结构，此微观结构和组成使材料诱导羟基磷灰石生成能力显著，利于成骨细胞黏附及繁殖，无细胞毒性，细胞兼容性好。通过植入兔股骨4和12周体内动物实验组织学切片表明，材料无毒、无刺激性，与周围组织间有良好的组织相容性，该材料植入动物骨环境中可刺激成骨，具有骨传导作用。相比下，微弧氧化和碱复合处理样品的生物相容性较为优异。%The surface morphology,coating composition,hydrophilicity deposition,cell adhesion and cytotoxici-ty of the titanium treated by microarc oxidation(MAO)and alkali were compared.The results showed the coat-ing treated by MAO-NaOH process showed a lot of cracks and honeycomb porous structure,and the coating was the porous reticular nano-structure with -OH polar group.The microstructure and composition could in-crease the surface hydrophilicity of materials.This would induce the rapid growth of hydroxyapatite.In vitro MG63 cell culture and MTT assay the MAO-NaOH samples showed distinct proliferation levels compared the other samples.It had no toxicity to the growth of MG63 cell and had better biocompatibility.After the titanium were implanted in rabbit femur for four weeks and the twelve weeks the histological sections showed materials were non-toxic,non-irritating,good histocompatibility with the surrounding tissue.The materials implanted animal bone environment could stimulate bone implanted and had bone conduction.Implanted experimental re-sults showed that all materials had good biological security and good tissue compatibility and samples treated by MAO-NaOH had the more excellent biocompatibility comparatively.