Modified badam shell was prepared on ultrasonic wave by bromohexadecyl pyridine (BHPD),and its adsorption capability for bilirubin was studied.Then a method for quantitative determination of bilirubin in dilute solution was developed by adsorption preconcentration and near-infrared diffuse reflectance spectroscopy (NIRDRS).The effects of pH,adsorption time and original concentration of bilirubin on adsorption were also investigated.After adsorbing bilirubin onto the adsorbent,NIR with diffuse reflectance mode were measured and partial least squares (PLS) model was established for fast quantitative prediction.The results showed that its adsorption capacity on modified badam shell was very obvious (above 96％) with vibration of ten minutes at neutral condition.The results of adsorption isotherm showed that the adsorption could be described by Langmuir model with a maximum adsorption of 10.74 mg/g for bilirubin.In addition,the NIR results showed that the correlation coefficient (R) between the predicted and the reference concentration was 0.9829 and the recoveries were in the range of 81.9％-117.9％ for the validation samples in the concentration range of 0.9-20.1 mg/L.Due to the facile synthesis process and good adsorption performance for bilirubin,the modified badam shell could be recommended as an adsorbent for enrichment and determination of bilirubin in dilute solution by NIRDRS combined with adsorption preconcentration in order to improve sensitivity and selectivity.%采用溴代十六烷基吡啶(BHPD)在超声条件下制备了改性巴旦木壳,以它作为吸附剂对胆红素的吸附行为进行了研究;再通过吸附预富集技术结合近红外漫反射光谱,建立了一种快速检测稀溶液中胆红素的方法.考察了pH值、吸附时间和胆红素初始浓度对吸附效果的影响;吸附了胆红素的吸附剂,通过近红外漫反射光谱直接检测,并采用偏最小二乘法建立定量预测模型进行定量分析.结果表明,在中性条件下,振荡吸附10 min,吸附率即可达到96％以上;吸附等温线研究结果表明,该吸附符合Langmuir等温吸附模型,对胆红素的最大吸附量为10.74 mg/g;近红外检测结果显示参考浓度和预测浓度之间的相关系数达到0.9829,胆红素在0.9～20.1 mg/L的浓度范围内,预测集样本的回收率可以达到81.9％～117.9％.鉴于BHPD改性巴旦木壳制备简便,对胆红素良好的吸附性能,可采用吸附预富集结合近红外漫反射光谱技术用于稀溶液中微量的胆红素的快速富集与灵敏检测.
展开▼
