杉木纤维素合酶(ClCesA2)基因的克隆与表达分析

摘要

In order to explore the mechanism of wood development of Chinese fir, total RNA extracted from Chinese fir seedlings was used for RT-PCR, then the full-length open reading frame sequence of ClCesA2 gene was amplified.Sequencing and analysis of the gene showed that the full length was 3276 bp, encoding 1091 amino acids, containing zinc finger structure, eight cross membranes and a conservative DX??QVLRW structure domain.The qPCR result revealed that the gene highly expressed in the stem, followed by root and leaf. Phylogenetic tree analysis showed that the gene was clustered with genes involved in the synthesis of the primary wall which inferred that the ClCesA2 gene may play an important role in the process of cellulose synthesis of the primary cell wall.%为探索杉木木材发育的分子机制,以杉木幼苗嫩叶总RNA为模板,利用反转录获得的cDNA进行聚合酶链式反应(PCR),获得杉木纤维素合酶(ClCesA2)基因.对该基因进行测序以及序列分析表明,该基因开放阅读框全长3276 bp,共编码1091个氨基酸,具有锌指结构和8个跨膜区域,及保守的DX??QVLRW结构域.荧光定量PCR结果表明,该基因在杉木的根、 茎、 叶中相对表达量有明显差异,茎的相对表达量最高,根次之,叶最低.系统进化树分析表明该基因与初生壁合成相关基因聚在一起,推测该基因可能参与杉木细胞初生壁的形成.