光照和氮源对朱砂根愈伤组织生长及岩白菜素含量的影响

摘要

通过调节光照条件及培养基中铵态氮与硝态氮的比例来研究光照和氮源对朱砂根愈伤组织生长及岩白菜素含量的影响.结果表明,遮光和强白光都有利于朱砂根愈伤组织的生长,不同光照度和光质对愈伤组织的生长形态和褐化程度有一定的影响.愈伤组织中的岩白菜素含量随着光照度的不断增强而减少,当光照度为100 lx(遮光状态)时,岩白菜素含量达到最高,为0.0169％.光质对岩白菜素含量影响程度的大小为:绿光＞蓝光＞白光＞红光,绿光下的岩白菜素含量最高,为0.0235％.培养基中的KNO3有利于愈伤组织生长,NH4NO3有利于岩白菜素的合成.当KNO3含量加倍但无NH4NO3时,愈伤组织的增殖系数会增大;当KNO3含量为3800 mg·L-1时,愈伤组织的增殖系数达到最大值,为19.9614;当培养基中仅有NH4NO3时,随着含量的增加愈伤组织的生长量和岩白菜素的含量都随之减少;当NH4NO3含量为1650 mg · L-1时,岩白菜素含量达到最高,为0.0125％.%This experiment researches into the effect of light and nitrogen source on the growth of callus and the content of bergenin in Ardisia crenata Sims by adjusting the proportion of ammonium and nitrate nitrogen in culture medium. The results showed that shading and strong white light were beneficial to the growth of the callus, light intensity and light type had certain effect on the growthform and degree of browning of the callus. The content of bergenin reduced with the increasing of light intensity . When the light intensity was 100 lx (shading) , the content of bergenin in the callus was highest(0.0169% ). The influence degree of light type on the content of bergenin was in the following order; green, blue, white, red. Under green light, the content of bergenin in the callus was highest(0.0235% ). The KNO3 was beneficial to the growth of the callus, and NH4NO3 was beneficial to the synthesis of bergenin. Under the condition of doubled concentration of KNO3 and lack of NH4NO3, the multiplication coefficient of callus may increase. When the concentration of KNO3 was 3800 mg · L-1, the multiplication coefficient of callus reached the maximum level(19.9614). When the culture medium only had NH4NO3, the mass growth and the content of byrgenin in the callus reduced with the increasing of the concentration . When the concentration of NH4NO3 is 1650 mg · L-1, the content of bergenin reached the maximum level(0.0125% ).