CRKL基因反义寡核苷酸对K562细胞增殖的影响

摘要

目的通过研究CRKL基因反义寡核苷酸对K562细胞增殖活性的影响，探讨CRKL基因在Ph+慢性粒细胞性白血病(CML)发病中的作用. 方法脂质体为载体，CRKL基因反义寡核苷酸转染K562细胞，通过活细胞记数、H3-TdR掺入、RT-PCR等方法观察K562细胞的增殖活性及基因表达的变化. 结果 CRKL反义寡核苷酸对K562细胞的增殖有抑制作用. [ HTH〗结论 CRKL基因在Ph+ CML的发病中可能有重要作用，可作为CML治疗的新靶点.%AIM　To investigate the effects of CRKL antisense-oligonucleutides (ASODN) on cell growth and proliferation in K562 cell line and thus assess CRKL gene in the pathogenesis of Ph+ CML. METHODS　K562 cells were cultivated with CRKL oligos which were mediated by cationin liposome. The cell viability, proliferation, and gene expression were examined by H3-TdR doping and RT-PCR . RESULTS　CRKL ASODN could inhi bit the viability and proliferation of K562 cells. CONCLUSION　CRKL gene may play a key role in the pathogenisis in Ph+ CML.