首页> 中文期刊> 《空军军医大学学报》 >大鼠多焦点视网膜电图的记录方法

大鼠多焦点视网膜电图的记录方法

         

摘要

The multi focal electroretinography (mERG)is a new technique to evaluate objectively the visual function and characterized by observing the function change in focal retina quantitatively. The recording condition is very rigorous and the relative position between the eye and stimuli field must be kept to obtain accurate results. In experiments, the albino Sprague Dawley rats were kept on a special platform, which can be moved in three dimensions, and the distance between the tested eye and CRT were controlled by a self made apparatus. A silver chloride loop electrode was contacted with corneal limbus as recording electrode. The waveforms of dark adapted mERG in albino rats were stable, clear and repeatable in focal retina. The amplitudes of a and b waves in upper field were greater than those in lower field while the latencies were shorter. There was no large amplitude area just like the human macular. This procedure paves the way for evaluating objectively functional change in focal retina in laboratory

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