利用前期制备得到的DON酶标抗原(辣根过氧化物酶标记)以及抗DON抗体,建立检测食品中DON含量的直接竞争ELISA方法.该方法的检测范围1～100 ng/mL,灵敏度达0.56ng/mL,半数抑制浓度IC50为10 ng/mL；与DON类似物T-2毒素的交叉反应率为12％；玉米淀粉样品回收率在80.2％～91.1％之间,平均批间变异＜15％,平均批内变异＜3％.%In this manuscript, the direct competition ELISA method for deoxynivalenol (DON) was established by using enzyme-labeled antigen of DON and antibody. It was found that the-detection range of this method is 1 --■ 100 ng/mL, sensitivity 0. 56 ng/mL, 50 % inhibitory concentration (IC50) 10 ng/mL, the cross-reaction rate to DON analogues T-2 toxin 12%, the recoveries of corn starch samples 80. 2%-91. 1%. Moreover, the average intra-assay variation and the average inter-assay variation is less than 15%, and 3%, respectively.
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