芽胞漆酶的固定化方法对比研究与载体选择

摘要

The comparative studies for immobilization of spore laccase were carried out using 19 different methods and 12 carriers.The optimal carrier and method were determined by the activity yield,reusability,and cost of the immobilized spore laccase.The ionic bonding method using DEAE-cellulose as the carrier was selected as the best method.The strong diffusion limitations were observed when the spore laccase was immobilized by embedding method.The microspheres might dissolve and swell if semi-embedding method was used.The small pore size made it difficult for porous materials to immobilized spore laccase.The excellent immobilization properties were achieved when the spores were covalently bound to the carriers using carbodiimide as the crosslinker.It was thus recommended as an alternative method.%为了找到适合芽胞漆酶固定化的载体和方法,对12种载体共19种芽胞漆酶固定化方案进行了对比研究,并通过参考固定化芽胞漆酶的酶活力回收率、重复利用率、成本等因素,选定以DEAE-纤维素为载体通过离子结合法固定为最佳方案.结果表明,包埋法固定化芽胞漆酶存在严重的扩散限制作用,部分包埋法制备的微球还会伴有溶解、膨胀的现象.而多孔材料常因孔径过小难以固定芽胞.芽胞以碳二亚胺为交联剂与载体进行共价结合,具有良好的固定化性能,可作为备选方案.