微反应器在酶催化合成哌啶甲酸中的研究

摘要

哌啶甲酸是哌啶环衍生物的重要前体.赖氨酸环化脱氨酶可以催化L-赖氨酸生产L-哌啶甲酸.为加快赖氨酸环化脱氨酶的催化反应速率,设计并制作了PDMS微芯片作为反应装置,并研究了微通道尺寸、微通道长度和反应液流速对反应速率的影响.在微反应器中最佳反应条件是:微通道尺寸200 μm;微通道长度128.4 cm;反应液流速100 μL/min.在优化的条件下反应44 h,获得哌啶甲酸2.22 g/L,比在摇管中反应所需时间缩短了1/3.%Pipecolic acid is an important precμrsor for piperidine ring derivatives.A lysine cyclodeaminase was used to catalyze L-lysine for L-pipecolic acid synthesis.To accelerate the catalytic rate,a PDMS microchip was designed and fabricated.Effects of microchannel size,microchannel length and liquid flow rate on catalytic rate were studied.The optimized conditions in microreactor were:microchannel size 200 μm;microchannel length 124.8 cm;and liquid flow rate 100 μm/min.Under the optimized conditions,a pipecolic acid titer of 2.22 g/L was obtained after 44 h reaction,the reaction time reduced 1/3 in comparison with the reaction in shaking tube.