全自动石墨消解-电感耦合等离子体质谱测定鱼、虾等生物样品中铜、镉、锌、铬4种重金属元素

摘要

Objective To develop an easy,fast,and accurate method for the simultaneous determination of Cu,Cd,Cr and Zn in biological samples like fish and shrimp.Methods Using 10.0 μg/L Rh as internal standard element,nitric acid,hydrogen dioxide and perchloric acid as digestion acids,fish,shrimp and other biological samples were digested by automatic graphite digestion instrument,and the method for the simultaneous determination of 4 metal elements of copper,cadmium,zinc and chromium by graphite digestioncombined with inductively coupled plasma mass spectrometry (ICP-MS) was established.Results The detection limits for each element were in range of 0.001～0.200 μg/L,the relative standard deviations (RSDs) ranged from 0 to 2.3％,and the recoveries were 91.4％～113.4％.The determined values of prawn (GSB-28)were in good agreement with the certified values.Conclusion The developed method is simple,rapid,sensitive and has good reproducibility,which is a reliable and efficient method for simultaneous determination of Cu,Cd,Cr and Zn in large quantities of biological samples.%目的 建立一种简便、快速的鱼、虾等生物样品中重金属元素的分析方法.方法 以10.0 μg/L的Rh作为内标,采用硝酸-过氧化氢-高氯酸体系,用全自动石墨消解仪消解鱼、虾等生物样品,建立石墨消解-电感耦合等离子体质谱法(inductively coupled plasma mass spectrometry,ICP-MS)同时测定样品中铜、镉、锌、铬4种金属元素的方法.结果 各元素的检出限在0.001～0.200 μg/L之间,相对标准偏差在0～2.30％之间,加标回收率为在91.4％～113.4％之间.对大虾标准品(GSB-28)进行分析的测定值与标准值吻合良好.结论 本研究所建立的方法简单、快速,灵敏度高,重现性好,是同时分析大批生物样品中多种金属元素的可靠、高效方法.