新型α-半乳糖苷酶清除动物红细胞表面αGal抗原的研究

摘要

αGal, a xenotransplantations antigen (XTA), can lead to hyper acute reaction (HAR) in xenotransplantation. A-Galactosidase from B. Fragilis is a novel galactosidase belong to CAZy GH110 which can clear the terminal αGal from branched and linear oligosaccharides. This study was purposed to investigate the removal effect of a novel a-galactosidase on a-Gal XTA on surface of red blood cells. The αGal XTA from the red blood cells of cattle, pig, dog and rabbit was digested by using recombinait a-galactosidase; the a-Gal antigens on surface of cells was detected by flow cytometry. The results showed that the XTA was disappeared completely or mainly. It is concluded that the novel a-galactosidase is a potential enzyme to remove the XTA on the surface of xenotransplants and can be used to overcome the HAR in xenotransplantation.%异种抗原αGal是引起异种移植免疫排斥反应的重要原因之一.脆弱类杆菌来源的α-半乳糖苷酶是一种新型的糖苷酶,能够切除支链多糖和直链多糖末端的αGal残基.本研究探讨新型α半乳糖苷酶清除红细胞表面αGal抗原的作用.利用新型的基因重组α-半乳糖苷酶酶解牛、猪、狗、兔红细胞上的异种抗原,并用流式细胞术分析细胞表面的αGal抗原.结果表明,动物红细胞表面的异种抗原αGal被完全或部分清除.结论:新型α-半乳糖苷酶可以用来清除异种红细胞上的αGal抗原,对降低异种移植引起的超急性排斥反应具有潜在意义.