A kind of psychrophile strain named pseudomonasflava WD-3 was isolated from the sediments of Nansihu Lake in winter,and it was used as the microbial carrier.The carbonized rice straw was prepared to be biocarbon as adsorbent,and the biochar-fixed Pseudomonas flava WD-3 bacteria was added into the vertical flow constructed wetland sewage treatment system to discuss the removal efficiency of nitrogen in wastewater of low temperature and to simulate the kinetics of degradation of pollutants in constructed wetland by a simplified Monod Model.The results demonstrate that the removal efficiency of nitrogen in waste water in constructed wetland was increased significantly,when hydraulic retention time was 5 d.The degradation kinetics of TN,NH4+-N,NO2--N,NO3--N were accorded with the Monod Model by biochar-fixed Pseudomonas flava WD-3.%以从冬季南四湖湿地底泥中分离筛选出的黄假单胞菌(Pseudomonas flava WD-3)为微生物载体,采用水稻秸秆炭化制备的优质生物炭为吸附剂,将生物炭固定化Pseudomonas flava WD-3投加到垂直流人工湿地污水处理系统中,探讨不同菌剂接种量对低温污水中氮素的去除情况;并采用简化的Monod动力学模型对人工湿地污水中污染物的去除过程进行模拟.结果表明,在水力停留时间5d时,生物炭固定化Pseudomonas flavaWD-3对人工湿地低温污水中氮素的去除效率有显著提高,且对人工湿地污水中TN、NH4+-N、NO3--N和NO2--N的降解符合Monod动力学模型.
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