采用反转录-聚合酶链式反应(RT-PCR)方法,以肝脏总RNA为模板,对泰和乌骨鸡、隐性白羽肉鸡腺苷琥珀酸裂解酶基因(ADSL)编码区序列进行克隆和测序；并结合Gen-Bank中已提交的家鸡、鱼类、哺乳动物和人类等ADSL基因序列,采用基于最大似然法的密码子置换模型分析ADSL基因编码区序列在进化过程中承受的选择压力.结果RT-PCR方法准确获得了泰和乌骨鸡和隐性白羽肉鸡ADSL基因编码区序列,全长为1 380 bp.“分支特异模型”检验结果表明,ADSL基因在不同物种进化过程中较为保守,未受到正选择作用.鸡ADSL基因序列的“位点特异模型”检验未发现正选择位点.研究结果有助于了解鸡ADSL基因的结构及进化历程.%The coding region of ADSL gene (Adenylosuccinate Lyase)was amplified from the extracted liver total RNA in Taihe Silkies and Recessive White Boilers , and were subcloned and sequenced in both directions. Some ADSL gene sequences that had been submitted in GenBank, including sequences of domestic chicken, fish, mammals and human were also used in this study. Maximum-likelihood models of codon substitution were used to analyze diversifying selection pressures of ADSL gene coding region. The results showed RT-PCR methods acquired the aimed fragments, in a total of 1380bp. "Branch-special model" test showed ADSL genes of different species were rather conservative, not having suffered positive selection pressure. "Branch-special model" also did not find positive sites in chicken ADSL genes. The results could help to trace the route of chicken ADSL gene evolution and knowledge of its structure.
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