齐墩果酸对体外培养人毛囊干细胞增殖的影响

摘要

Objective:To investigate the effect of oleanolic acid (OA) on proliferation of human hair follicle stem cells (HFSC).Methods:HFSC were isolated and cultured in vitro and identified by fluorescent staining.OA was added to medium at final concentration of 1×10-4 μg/mL,1×10-3 μg/mL, 1×10-2 μg/mL, 0.1 μg/mL and 1 μg/mL, then MTT assay was performed when the cell culture was treated with OA at 24 h and 48 h to assess the number of viable cells.Results:Cultured cells were multipolar in shape and grew like typical cobble stone.The staining of K15 was positive, which is an epithelial stem cell marker.Positive rate of K15 is (86.7±5.2)% (n=4).Compared to the vehicle control group, OD490 of 1×10-4 μg/mL and 1×10-3 μg/mL of OA treated group were significantly increased (P<0.05).Conclusion:OA could stimulate proliferation of human hair follicle stem cells in vitro.%目的:探究齐墩果酸对人毛囊干细胞增殖活性的影响.方法:取术中毁弃的人全层头皮标本,使用两步酶消化法分离提取毛囊干细胞进行体外培养,运用免疫荧光K15染色鉴定;将细胞以5×104个/mL的密度接种于96孔板,培养液中分别加入1×10-4、1×10-3、1×10-2、0.1、1 μg/ mL梯度浓度齐墩果酸,于处理的24 h及48 h使用MTT法检测毛囊干细胞活性.结果:获得的毛囊干细胞呈典型的铺路石状生长,免疫荧光K15阳性,阳性率为(86.7±5.2)% (n=4).与空白组相比,1×10-4μg/mL及1×10-3μg/mL齐墩果酸处理组在24 h及48 h的OD490均有明显升高(P<0.05).结论:齐墩果酸能够促进体外培养的人毛囊干细胞增殖.