Fluorescence method under different conditions was used to study the process of myoglobin deoxy, the ability of myoglobin oxygen bonding was gradually increasing as the temperature raised; when the pH of solution changed, oxy-myoglobin was more easier to be deoxy-myoglobin under acidic condition than under alkaline condition; reducing the pressure would help oxy-myoglobin to be the deoxy-myoglobin; On the contrary, increasing the pressure helped myoglobin combine with oxygen; when the myoglobin solution passed into CO2, ventilation must, with the reaction time changing, could be drawn, the shorter the reaction time more conducived to myoglobin combined with oxygen, with the reaction time increasing, oxy-myoglobin gradually became deoxy-myoglobin; when the solution passed into CO, CO combined with the Fe(Ⅱ) could deoxy myoglobin.% 利用荧光法研究不同条件下肌红蛋白去氧过程，发现肌红蛋白随着温度的升高结合氧的能力逐渐增加；在改变溶液酸碱性的条件下，肌红蛋白在酸性条件下比在碱性条件下更容易去氧；减小压力有助于肌红蛋白去氧；相反，增加压力有助于结合氧；肌红蛋白溶液通入 CO2，通气量一定，随反应时间延长，可以得出，在短时间内，CO2利于肌红蛋白结合氧，随着反应时间再增加，肌红蛋白又逐渐去氧；在通入 CO 时，CO 与肌红蛋白中的 Fe(II)结合，可使肌红蛋白去氧。
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