肺癌细胞株E - cadherin的表达状态及其与启动子甲基化的关系

摘要

[Objective] To investigate the relationship between the promoter methylation status and protein expression of E - cadherin in lung cancer cell lines. [ Methods] The protein expressions of E - cadherin in lung cancer cell lines NCI -H460,SPCA and A549 were examined by immunocytochemistry. The promoter methylation status of E - cadherin was detected by methylation - specific PCR ( MSP) and microfluidics. [ Results] The weak expressions of E - cadherin in NCI -H460,SPCA and A549 were detected, the weakest in A549. The promoter methylation of E - cadherin occurred in all three cell lines and the highest percentage of methylation in A549, which was in agreement with the protein expression. [ Conclusions] The promoter methylation can be a cause of E -cadherin its down - regulation in lung cancer cell lines.%[目的]研究肺癌细胞株上皮钙粘蛋白(E - cadherin)的表达与基因启动子甲基化状态的关系.[方法]采用免疫细胞化学法检测肺癌细胞株NCI - H460、SPCA和A549的E- cadherin蛋白表达；半巢式甲基化特异性PCR法(MSP)和微流控芯片技术检测E- cadherin基因启动子甲基化状态.[结果]E- cadherin蛋白在NCI - H460、SPCA和A549肺癌株均呈弱表达,以A549细胞株表达最低；MSP法和微流控芯片技术在三种细胞株中均检测到E - cadherin基因启动子的甲基化,以A549细胞株启动子甲基化程度最高,二者结果相符.[结论]E- cadherin基因启动子甲基化可能是肺癌细胞株NCI - H460、SPCA和A549出现E- cadherin蛋白表达下调的原因.