水通道蛋白-1在急性肺损伤中的表达及意义

摘要

[Objective] To investigate the expression and effect of AQP - 1 in acute lung injury ( ALI) induced by lipopo-lysaccharide. [Methods] Twenty Sprague - Dawley rats were randomly divided into LPS group treated with LPS injection and normal control group injected with saline (Control group). Arterial oxygen tension ( PaO2 ) and wet/dry mass ratio ( W/D) were tested at 6th hour after the injection. The expressions of AQP - 1 protein in the lungs of rats were detected by immunohistochemistry and the expressions of AQP - 1 mRNA were measured by a real - time fluorescence PCR. [ Results] The arterial oxygen tension ( PaO2 ) in LPS group was obviously lower than that in control group (55. 07 ±17. 02) mmHg vs (97.35 ±9.37) mmHg (P<0. 05). The wet/dry mass ratio (W/D) in LPS group was obviously higher than that in control group (4.93±0.16vs 4.11 ±0. 69, P<0.05). The immunostaining showed a significant decrease in the expression of AQP - 1 and a real time RT - PCR confirmed that the levels of AQP - 1 mRNA were significantly reduced in LPS group (P < 0. 01) at 6 h after LPS injection. [ Conclusion] AQP - 1 has an great effect in acute lung injury ( ALI) .u0000ecreu0000se i%[目的]探讨水通道蛋白1(AQP-1)在脂多糖(LPS)所致的急性肺损伤(ALI)中的作用.[方法]根据是否腹腔注射脂多糖(LPS)将实验动物分为脂多糖组(LPS组)及生理盐水对照组(Con组),对比两组动物组织病理学改变、动脉血气、肺湿/干重比及AQP-1表达的差异.[结果]光镜下见LPS组肺组织水肿,大量炎性细胞浸润；LPS组P02(55.07±17.02)mmHg明显低于Con组(97.35±9.37) mmHg,差异有显著性意义,P＜0.05；LPS组肺湿/干重比(4.93±0.16)明显高于Con组(4.11±0.69),差异有显著性意义,P＜0.05；免疫组化染色显示:LPS组的AQP-1蛋白表达减弱,荧光实时定量PCR证实LPS组的AQP-1 mRNA表达较对照组明显下降(P＜0.01).[结论] AQP-1参与了急性肺损伤的形成.