Objective To observe the morphological changes of enteric nerve and ICC of enteric nerve -ICC-smooth mus-cle cells network in rats with Yang Ming Fu Shi Syndrome , and to investigate the function of gastrointestinal motility and the therapeutic Mechanism of Xa -Fa in Traditional Chinese .Methods One handred Wistar rats of both sexes were randomly divided into three groups , the contrpl group 20 rats, the model group 40 rats and the therap group 40 rats.The proximal 10.0 cm segment of jejunum beginning 2 cm distal to the pylorus from each group , was studied using c-Kit and vesicular acetylcholine transporter ( VAChT)euronal nitric oxide synthase ( nNOS) immunohistochemical double -staining with whole-mount preparation technique and confocal laser scanning microscopy .Results Change of enteric nerve -ICC net work with confocal laser scanning microscopy:Model group:Compare with control group , the numbers of ICC were signifi-cantly decreased (P <0.01), network was disrupted, fluorescence intensity of ICC decreased.Compare with control group, the numbers of vesicular acetylcholine transporter ( VAChT) and neuronal nitric oxide synthase ( nNOS) were sig-nificantly decreased (P<0.01), nerves network disordered, each connection decreased, fluorescence intensity of enteric nerve decreased(P<0.01).The connection of ICCs and enteric nerves were loosen or disappeared .ICC-enteric nerve network was significantly disrupted .Treat group: Compare with control group , the numbers of ICC were significantly in-creased (P<0.01), network was recovered, fluorescence intensity of ICC increased.the numbers of vesicular acetylcho-line transporter (VAChT) and neuronal nitric oxide synthase (nNOS) were significantly increased( P<0.01), network was recovered, fluorescence intensity of nerves increased (P<0.01).The connection of ICCs and enteric nerves were ap-proaching normal , ICC-enteric nerve network was significantly recovered .Conclusion Gastrointestinal tract distended in Yang Ming Fu Shi Sydrom rats .Function of gastrointestinal was significantly disordered;and recoveried after Da Cheng Qi decoction treated.In Yang Ming Fu Shi Sydrom rats, numbers of vesicular acetylcholine transporter (VAChT) and neuronal nitric oxide synthase ( nNOS) were significantly decreased;and numbers of ICC were decreased too .entric nerve-ICCnet-work was disrupted;after treated, numbers of nerves and ICC were increased ,and network was recoveried .%目的：观察阳明腑实证大鼠小肠神经-Cajal 间质细胞（interstitial cells of Cajal，ICC）-平滑肌细胞网络的形态学变化，探讨阳明腑实证胃肠运动障碍的产生机制及下法的作用机制。方法成年Wistar大鼠100只，雌雄不限，随机分成3组，对照组20只；阳明腑实证模型组40只；大承气汤治疗组40只。存活大鼠取上段小肠10．0 cm组织制作肠肌层全厚组织标本，进行c-Kit和乙酰胆碱转运体（VAChT）/一氧化氮合酶（nNOS）免疫荧光双标记染色后用于激光扫描共聚焦显微镜检测、分析。结果（1）共聚焦显微镜检测ICC网络：与对照组相比，模型组大鼠小肠ICC数量明显减少（P＜0．01），ICC突触的数量减少，相互间的连接不连续，完整的网络样结构消失，细胞荧光强度减弱（P＜0．01）。（2）共聚焦显微镜检测肠神经-ICC网络：与对照组相比，模型组胆碱能/氮能神经数目明显减少（P＜0．01/P＜0．01），神经网结构紊乱，彼此间的连接减少，神经网络的荧光强度减弱（ P＜0．01/P＜0．01）；ICC与肠神经纤维两者间失去紧密连接，肠神经-ICC网络的完整结构受到破坏。（3）治疗组较模型组ICC数量明显恢复（P＜0．01），网络样结构的完整性有所恢复，与模型组比较细胞荧光强度明显增强（P＜0．01）。胆碱能神经/氮能神经纤维数目明显增加（P＜0．01），神经网络结构再次好转，彼此间的网络连接的完整性恢复明显，神经网络的荧光强度增加（P＜0．01），ICC与肠神经纤维间的连接接近正常，ICC-肠神经网络结构恢复明显。结论阳明腑实证大鼠小肠ICC和神经网络受到破坏。大承气汤治疗后小肠胆碱能/氮能神经和ICC数量恢复，肠神经-ICC网络结构得到改善。
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