中药葛根微生物转化菌株的鉴定及转化特性的研究

摘要

从中药葛根的微生物转化试验中,获得1株能够较好转化葛根有效成分的丝状真菌,编号为YM3207,通过对该菌的形态特征观察及ITS序列分析,确定该菌为紫色红曲霉Monascus purpureus.经HPLC定量分析显示,转化后染料木素和葛根素含量得到提高;同时,抑菌活性较转化前有一定程度的增强,抗菌谱变广.%A strain of filamentous fungi which was able to transform the active ingredient in Puerariae radix was obtained in microbial transformation experiments and named YM3207. After observing and analyzing the morphology and ITS sequence of this fungi, it was identified as Monascus purpureus. HPLC was used to analyze the change of transformation column. The content of Genistein and Puerarin have been improved. At the same time, antibacterial activity enhanced and antimicrobial spectrum expanded as well.