Th17细胞在严重全身性感染患者外周血中的表达及临床意义

摘要

目的：检测严重全身性感染患者外周血Th17／CD4＋T细胞的比例及单个核细胞（ PBMC）中RORγtmRNA和IL-17 mRNA的表达水平，探讨Th17细胞在严重全身性感染患者外周血中的临床意义。方法：采用流式细胞分析法检测20例严重全身性感染患者、20例感染性休克患者及20例健康体检者外周血Th17／CD4＋T细胞比例，RT-PCR法检测PBMC中RORγt mRNA及IL-17 mRNA的水平。结果：严重全身性感染组及感染性休克组外周血Th17／CD4＋T细胞比例、RORγtmRNA及IL-17 mRNA水平均高于健康对照组，且严重全身性感染组高于感染性休克组（均P＜0．05）。结论：严重全身性感染及感染性休克患者外周血Th17／CD4＋细胞比例增加，RORγt及IL-17蛋白表达水平升高，Th17细胞参与了炎症的发生、发展。%Objective:To detect the Th17/CD4 +T cells ratio in peripheral blood and the expression of RORγt mRNA and cytokine IL-17 mRNA in peripheral blood mononuclear cells ( PBMC) in patients with severe sepsis , and to explore its clinical significance .Methods:Flow Cytometry ( FCM) was used to detect the peripheral blood ratio of Th 17/CD4 +T cells in 20 cases of severe systemic infection , 20 cases of septic shock patients and 20 healthy subjects , reverse transcription PCR ( RT-PCR) was used to detect the expressions of RORγt and cytokine IL-17 mRNA in PBMC of all groups .Results:The ex-pressions of the peripheral blood ratio of Th 17/CD4 +T cells and RORγt mRNA and cytokine IL-17 mRNA in PBMC of pa-tients with severe systemic infection and septic shock were higher than those of healthy control group .The expressions of the peripheral blood ratio of Th 17/CD4 +T cells and expressions of RORγt mRNA and cytokine IL-17 mRNA in PBMC in se-vere systemic infection were higher than those of septic shock group (P<0.05).Conclusions:The expression of the periph-eral blood ratio of Th17/CD4 +T cells in patients of severe systemic infection and septic shock , and its expressions of RORγt and cytokine IL-17 mRNA in PBMC are increased , all these suggest that Th 17 cell may involve in occurrence and develop-ment of inflammation .