Objective To investigate the appropriate dilution times for samples of high concentration of HBsAg in quantitative determination. Methods 122 cases of samples with high concentration of HBsAg were diluted with the different levels of serum dilution times. Results 86.1% of the specimen were successfully detected after successfully 101 diluted times, 96. 7% of the specimen were successfully detected after diluted 201 times, 99. 2% of the specimens were successfully detected after diluted 301 times, 100. 0% of the specimens were successfully detected after diluted 601 times. These revealed the successful rate of the assay increased along with the diluted times. Conclusion When the concentration of HBsAg exceeds 250 IU/ml, it should be detected after 300 times of dilution.%目的 探讨定量检测HBsAg时,高浓度HBsAg的最适稀释倍数,用以提高检测准确度.方法 对122例高浓度HBsAg患者的血清按照不同的稀释倍数稀释后进行测定.结果 86.1％的标本稀释101倍后能检测出结果,96.7％的标本稀释201倍后能检测出结果,99.2％的标本稀释301倍后能检测出结果,100.0％的标本稀释601倍后能检测出结果.随着稀释倍数的增加,所得检测结果的数值在增加.结论 当HBsAg＞250 IU/ml时,建议选择300倍稀释后检测.
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