目的：分析ELISA法不同S/CO值与金标法HBsAg检测结果的相关性，以科学方式指导血液采集，减少血液资源的浪费。方法选择2014年11月~2015年4月邯郸地区无偿献血者37966例。经采血车初筛合格后，对前3个月无偿献血者ELISA法检测阳性标本在实验室内再使用金标法进行复测，对ELISA和金标法检测结果进行对比分析。结果2014年11月~2015年1月共检出无偿献血者HBsAg阳性标本102例，阳性率为0.52％，其中科华和万泰检出率分别为90.19％和86.27％，差异无统计学意义（χ2=0.76，P＞0.05）；而金标法检出率较低为51.96％，分别与科华、万泰比较，差异均具有统计学意义（χ2=33.33，P<0.01；χ2=25.47，P<0.01）；两种酶免试剂检测S/CO值20以下的标本再用金标法检测，漏检率均为100.00％，科华和万泰S/CO值20以上的标本再用金标法检测，漏检率分别为12.69％和11.29％；2014年11月~2015年4月各月HBsAg阳性报废率呈逐渐降低趋势，有统计学意义（χ2=14.58，P<0.05）。结论改善金标法检测环境、使用血清加样，以及提高血液初筛人员的操作能力和工作责任心，是降低血液HBsAg阳性报废率的有效途径。%Objective To Analyze the correlation of HBsAg detection results by ELISA with differentS/CO values and gold-labeling methodfor safe collection of blood and reduction of the waste of blood resources.Methods37966 volunteer blood donors were subjected to detection in Handan area from Nov2014to Apr2015. After primary screening of HBsAg,gold-labeling method was used for re-detection of the samples that showed positive HBsAg in the previous detection by ELISA. The results of ELISA test were compared with those of gold-labeling.Results From Nov2014 to Jan 2015,HBsAg was found to be positive in102 cases of volunteer blood donors,with a positive rate of0.52%. The gold-labeling detection rate seemed to be various in companies(χ2=33.33,P<0.01;χ2=25.47,P<0.01). The false positivity was noted when re-tested by gold-labeling in all of the apecimens below theS/CO value of20,Hereafter,the HBsAg- positive rate showed a gradual reduction(χ2=14.58,P<0.05).Conclusion It is an efficient way for reduction of false positivity of HBsAg to improve the gold-labeling method and operation skills of technicians.
展开▼
