链脲佐菌素诱导C57BL/6小鼠糖尿病瘙痒模型的建立

摘要

[Objective]To investigate the optimal dosage of STZ (streptozotocin) in inducing diabetic itch in C57BL/6 mice.[Methods]Twenty-four male aged 8 weeks old C57BL/6 wild type mice (the weight 20～28 g) were randomly divided into 4 groups.A.Control group (mice were given the same dose solvent by intraperitoneal injection);B.High dose group (mice were intraperitoneally injected with STZ 160mg/kg);C.Low dose group (mice were intraperitoneally injected with STZ 40mg/kg 5 times continuously and the interval time was 24 hours);D.Mild moderate dose group (mice were intraperitoneally injected with STZ 85mg/kg + 65mg/kg and the interval time was 24 hours).Mice in the Group A were fed with normal food and B、C、D groups mice were fed with high-fat food from 4 weeks before the injection to the end of experiment.At the time of 1D before the injection and 1,2,3,4 weeks after injection, the fasting blood glucose, body weight and scrathing bouts in 30 minutes were detected.[Results]Compared with the group A, the blood glucose level of other groups increased to the diabetic standard(>200mg/dl)at different time point (P<0.05).The weight of the group B was lower than that of the group A, group C and group D (P<0.05), and the number of scratching in the group D was increased more than that of A, B and C at second, third weeks after STZ injection (P<0.05).[Conclusions]It is a suitable way to establish the diabetic itch model of mice induced by intraperitoneal injection 85mg/kg+65mg/kg STZ combining with feeding high-fat food.%[目的]探讨链脲佐菌素(streptozotocin , STZ)诱导C57BL/6小鼠糖尿病瘙痒模型的最佳剂量.[方法]24只雄性8周C57BL/6野生型小鼠,体重20～28 g,随机分为四组: A组(对照组)单次腹腔注射等剂量柠檬酸钠溶液 10 mL/kg,pH=4.5;B组STZ单次大剂量腹腔注射STZ 160 mg/kg;C组STZ腹腔注射40 mg/kg,连续注射5 d,每次给药时间间隔24 h;D组STZ两次中剂量注射,腹腔注射STZ 85 mg/kg+65 mg/kg,两次间隔时间24 h.从给药前4周开始,A组正常饮食,B、C、D组高脂饮食喂养至实验结束.注射STZ前1 d、注射STZ后1、2、3、4周测定动物的空腹血糖(FBG)、体重,采用录像记录30 min搔抓次数.[结果]与A组相比,B、C、D组造模后血糖升高并在不同时间点达到糖尿病标准(>200 mg/dL)(P<0.05),B组体重较A、C、D组下降(P<0.05),D组在STZ注射后第2、3周搔抓次数较A、B、C增多(P<0.05).[结论]腹腔分两次、间隔24 h注射STZ 85 mg/kg+65 mg/kg并配合高脂饮食是建立糖尿病并发瘙痒小鼠模型的合适方法.