对硝基苯甲酸鉴别结核分枝杆菌复合群与非结核分枝杆菌的机制研究

摘要

Objective To investigate the mechanism of identification of Mycobacterium tuberculosis complex (MTC)from non-tuberculous Mycobacterium(NTM)by p-nitrobenzoic acid(PNB). Methods 5 MTC reference strains and 33 NTM reference strains were included in this study. Metabolites of PNB reduction were identified using liquid chromatography and tandem mass spectrometry(LC/ MS/ MS). Results All of the tested NTM reduced PNB to p-aminobenzoic acid(PABA),while none of the MTC strains showed a similar activity. Conclusion PABA is i-dentified as one of the metabolites of PNB reduction. All the tested NTM species metabolize PNB to PABA whereas the MTC members lack this activity,which may be the mechanism of PNB inhibition test.%目的：研究对硝基苯甲酸（PNB）鉴别结核分枝杆菌复合群（MTC）与非结核分枝杆菌（NTM）的机制。方法应用高压液相色谱（HPLC）和液相色谱串联质谱法（LC/ MS/ MS）鉴定5株 MTC 标准株和33株NTM 标准株的 PNB 代谢产物。结果33株 NTM 标准株均可将 PNB 还原为对氨基苯甲酸（PABA），而5株MTC 标准株则不具备该能力。结论 PABA 是 PNB 的代谢产物之一。NTM 能将 PNB 还原为细菌可利用的PABA，因此能够耐受 PNB；MTC 不具备该能力，因而不能耐受 PNB。这可能就是 PNB 选择性培养基用于鉴别MTC 与 NTM 的机制。