Cullin7在肺癌组织中的表达及临床意义

摘要

Objective To study the expression of Cullin7 in lung adenocarcinoma and its clinical signifi-cance. Methods From March 2014 to January 2016 in our hospital,54 cases of lung adenocarcinoma tissue and tis-sue adjacent to carcinoma specimens by surgical resection of primary lung adenocarcinoma patients were collected for experiment. Cullin7 of tissue samples were detected by immunohistochemistry, RT-PCR and cell transfection meth-ods. Results Cullin7 mRNA expression quantity in lung tissue and in normal tissue adjacent to carcinoma were re-spectively(13.51 ± 4.30) ng/mL and(6.54 ± 2.14) ng/mL. Cullin7 mRNA expression level in lung cancer tissues was obviously higher than that in normal tissue adjacent to carcinoma,and there was statistically significant difference (t=4.69,P=0.000,P<0.01). The Cullin7 protein positive cells was significantly higher in lung cancer tissues than in normal tissues adjacent to carcinoma(t= 4.54,P=0.000,P<0.01). The level of Cullin7 mRNA in lung cancer tissue had nothing to do with age or type of lung cancer patients. With the increase of pathological stage and tumor size,and the decrease of differentiation,the expression of Cullin7 mRNA enhanced. In addition, the expres-sion of Cullin7 mRNA in lung cancer tissues was related with lymph node metastasis,and it was higher in lymph node metastasis(P<0.05). When Cullin7 expression was restrained,the expression of A427, H460, A549 and H1299 decreased significantly (P<0.01). Conclusion Cullin7 expression in lung cancer is higher than in normal tissue adjacent to carcinoma. The high expression of Cullin7 is related to lung tissue pathological stage,tumor size,differ-entiation degree and lymph node metastasis,which may become a target gene for targeted therapy of lung cancer.%目的 探讨Cullin7在肺癌中的表达及临床意义.方法 收集2014年3月至2016年1月期间被我院收治的54例肺癌患者手术切除的肺癌组织和癌旁组织为实验标本.采用免疫组化、RT-PCR、细胞转染等方法检测组织标本中的Cullin7表达情况.结果 肺癌组织、癌旁正常组织中Cullin7 mRNA表达量分别为(13.51 ± 4.30)ng/mL、(6.54 ± 2.14)ng/mL,肺癌组织中Cullin7 mRNA表达水平明显高于癌旁正常组织,差异有统计学意义(t=4.69,P=0.000,P<0.01).肺癌组织Cullin7蛋白染色阳性细胞数明显高于癌旁正常组织,差异有统计学意义(t=4.54,P=0.000,P<0.01).肺癌组织中Cullin7 mRNA表达量与患者年龄、肺癌类型无关.随着肺癌病例病理分期的升高、肿瘤大小的增加、分化程度的降低,肺癌组织中Cullin7 mRNA表达增强;此外,肺癌组织中Cullin7 mRNA表达量与病例是否伴淋巴结转移有关.伴淋巴结转移的肺癌组织, Cullin7 mRNA表达量高于未出现淋巴结转移的肺癌组织(P<0.05).Cullin7表达受到抑制后,A427、H460、A549和H1299的表达明显减少(均P<0.01).结论 Cullin7在肺癌组织中的表达高于癌旁正常组织.Cul-lin7的高表达与肺癌组织的病理分期、肿瘤大小、分化程度及淋巴结转移有关.Cullin7有望成为肺癌靶向治疗的目标基因.