哮喘小鼠CC16表达及糖皮质激素对其的影响

摘要

目的 观察哮喘小鼠气道Clara细胞主要分泌蛋白CC16表达以及布地奈德干预后对其的影响,探讨CC16在哮喘发病中的作用.方法 72只健康小鼠随机分为3组:即哮喘组、布地奈德干预组和正常对照组.用卵蛋白致敏、雾化建立小鼠哮喘模型,用RT-PCR方法检测肺组织CC16 mRNA表达,并观察其在各组间的变化.结果 哮喘组小鼠肺组织CC16 mRNA表达均明显低于正常对照组(P ＜ 0.05);经布地奈德干预后,肺组织CC16 mRNA表达较干预前明显升高(P ＜ 0.05).结论 CC16参与哮喘发病,可能是其中一种重要的内源性抗炎保护因子;而糖皮质激素在哮喘中的抗炎作用部分可能是通过提高血清CC16水平而实现.%Objectives To investigate the Clara cell secretory protein (CC16) expression in the blood and lung tissue of asthmatic mice and the effect of budesonide on CC16 expression, and to analyze its role in pathogenesis of asthma.Methods Total 72 healthy mice were divided randomly into three groups: allergic asthma group, budesonide group and normal control group.The asthma model of mouse was established by sensitized and challenged with ovalbumin.RT-PCR was performed for CC16mRNA in the lung tissue and expression of CC16mRNA among the three groups was compared.Results The expression of CC16mRNA is significantly decreased in the lung of asthmatic mice, compared with the normal control group (P ＜ 0.05) ; it is significantly increased with the treatment of budesonide, compared with the allergic asthma group (P ＜ 0.05).Conclusions It is suggested that CC16 may be an important endogenous anti-inflammatory factor and involved in asthma develpment.Glucocorticoids may play anti-inflammatory role in treatment of asthma by increasing CC16 in serum.