hsa-miR-1908靶基因预测及生物信息学分析

摘要

Objective To predict the biological process and signaling pathways in which hsa-miR-1908 might be in-volved by a series of bioinformatics analysis, so as to lay foundations and provide theoretical basis for the further studies of hsa-miR-1908 biological function in human preadipocytes. Methods The sequence of hsa-miR-1908 was acquired from miR-Base database, and target genes of hsa-miR-1908 were predicted by miRanda, and then the intersection of the results and the results of gene-chip as gene set were further analyzed by gene ontology and pathway enrichment. Results The hsa-miR-1908 had some conserved property among different species. The functions of the target genes were enriched in Wnt receptor signal-ing pathway through beta-catenin, cell cycle, cell apeptosis and other biological processes. The GnRH signaling, MAPK sig-naling, insulin signaling, cell cycle signal transduction pathways and signaling pathway in pancreatic cancer were signiifcantly enriched. Conclusions The target genes set of hsa-miR-1908 were enriched in multiple biological process which are related with the obesity. This study provides guidance for the further study in human preadipocytes.%目的对hsa-miR-1908进行系统的生物信息学分析，预测其可能参与的生物学过程及信号通路，为深入研究其在人脂肪细胞分化、肥胖发生等过程中的功能与机制奠定基础。方法应用miRBase获取hsa-miR-1908的序列，并分析其特征；应用miRanda预测hsa-miR-1908的靶基因，并取预测结果及基因芯片结果的交集，进一步进行基因功能注释（Gene ontology）和生物通路富集分析（Pathway enrichment)。结果 hsa-miR-1908在各物种之间有一定保守性，其靶基因功能富集于Wnt受体信号的调控、细胞周期、细胞凋亡等生物学过程；其靶基因信号通路富集于促性腺激素释放激素信号通路、MAPK信号通路、胰岛素信号通路、细胞周期等信号转导通路及胰腺癌等疾病通路中。结论 hsa-miR-1908预测的靶基因集合富集于多个信号通路及生物学过程，与肥胖密切相关，为后续has-miR-1908在人脂肪细胞中生物学功能研究奠定基础。