乌鲁木齐地区维、汉族儿童哮喘与ADAM33基因多态性的相关性

摘要

Objective To explore the correlation between ADAM33 SNP and asthma in Uygur and Han children in Urumqi. Methods Eighty-six Uygur and 111 Han children aged 3-15 years old who had asthma and lived in Urumqi were included. Meanwhile 56 Uygur and 64 Han healthy children were also included as control group. Genotyping of single nucleotide polymorphism (SNP) of V4 and T2 loci in ADAM 33 gene was performed by PCR, and verifications was made. Results There were statistical differences of V4 and T2 loci in ADAM 33 gene among asthma group and control group (P all < 0.01); CC genotype frequency was high in V4 loci in asthma group; Compared with G allelic gene, C allelic gene caused a 1.51-fold increased risk of asthma (95%CI:1.10-2.09). AA genotype frequency was high in T2 loci in asthma group; Compared with G allelic gene, an allelic gene caused a 1.96-fold increased risk of asthma (95%CI:1.32-2.91). There was no difference of V4 or T2 loci in ADAM 33 gene between asthma group and control group in Han children (P all > 0.05) while there were differences in Uygur children (P all< 0.05). Conclusions V4 or T2 loci in ADAM 33 gene is associated with asthma in Uygur children.%目的：探讨乌鲁木齐地区维、汉族儿童支气管哮喘与ADAM33基因多态性的相关性。方法选取同期3~15岁于乌鲁木齐地区生活的86例维族和111例汉族哮喘患儿，同时纳入64例汉族、56例维族健康儿童为对照组；采用聚合酶链反应对ADAM33基因V4、T2位点进行单核苷酸多态性（SNP）分型，并对其中部分进行基因检测验证。结果哮喘组和对照组之间，ADAM33基因V4、T2位点三种基因型的分布差异有统计学意义（P均 < 0.01）；V4位点哮喘组CC基因型频率较高，与G等位基因个体比较，C等位基因携带者儿童发生哮喘的风险增加1.51倍（95% CI：1.10~2.09）；T2位点哮喘组AA基因型频率较高，与G等位基因个体比较，A等位基因携带者儿童发生哮喘的风险增加1.96倍（95%CI ：1.32~2.91）。汉族儿童中，哮喘组和对照组之间 ADAM 33基因 V 4、T 2位点三种基因型分布的差异无统计学意义（P 均 > 0.05）；而维族儿童中，V 4、T 2位点三种基因型分布的差异有统计学意义（P 均 < 0.05）。结论 ADAM 33基因V4、T2位点与乌鲁木齐地区维族儿童哮喘发病相关。