E2F1蛋白在胶质瘤中的表达及其对胶质瘤细胞生长的影响

摘要

目的：探讨E2 F1蛋白在胶质瘤中的表达及其对胶质瘤细胞生长的影响。方法用实时荧光定量PCR法检测E2F1mRNA在各级别胶质瘤组织中的表达水平。应用RNA干扰技术降低E2F1表达后，采用Western印迹法验证E2F1蛋白的变化。采用四甲基偶氮唑盐（ MTT）微量酶反应比色法和糖检测分析试剂盒检测干扰E2 F1表达后胶质瘤细胞的增殖和糖代谢的变化。结果胶质瘤组织中E2 F1的表达高于正常脑组织，其表达随着级别的增高而升高；抑制E2F1的表达后，U87细胞生长受抑制，细胞的葡萄糖消耗量明显降低。结论 E2 F1在胶质瘤中的表达随着肿瘤恶性程度的增高而升高；抑制E2 F1表达可以明显抑制胶质瘤细胞增殖及代谢能力。%Objective To study the expression of E 2 F1 protein in human glioma tissues and its effect on tumor growth .Methods E2F1 expression in glioma tissues was determined by real-time quantification RT-PCR.Subsequently, MTT assay, western blotting and Glucose Assay were used to assay U87 cells with reduced E2F1 expression.Results This data of the study showed that the expression of E2F1 in glioma was increased and strongly correlated with its pathological grade . Abrogating expression of E2F1 could suppress glioma cells growth , decrease the glucose consumption.Conclusions The expression levels of E2F1 mRNA in human glioma is high. Abrogating expression of E 2F1 could inhibit the growth and metabolism in U 87 cells.