端粒结合蛋白-1在脑胶质瘤中的表达及临床意义

摘要

目的：探讨端粒结合蛋白-1（TRF-1）在不同级别脑胶质瘤及正常脑组织中的表达及其临床意义。方法选取58例脑胶质瘤和10例脑外伤内减压脑组织石蜡切片标本作为研究对象，应用免疫组化技术检测各组TRF1的表达水平，应用半定量法计算不同标本肿瘤细胞免疫标记的频率和强度积分。结果共58例不同级别胶质细胞瘤，其中低度恶性组（WHOⅠ、Ⅱ级）27例，高度恶性组（WHOⅢ、Ⅳ级）31例，10例脑外伤病人内减压脑组织作为正常对照。在所有胶质瘤组织及正常脑组织中均检测到 TRF1表达，其中正常脑组织中TRF1表达高积分构成比90．00％，低度恶性胶质瘤组（WHOⅠ、Ⅱ级）的TRF1表达高积分构成比62．96％，高度恶性胶质瘤组（WHOⅢ、Ⅳ级）29．03％。统计分析发现，TRF1在不同级别胶质瘤中的表达与其恶性程度成负相关。结论在人脑胶质瘤和正常脑组织均有TRF1的广泛表达，其在不同级别脑胶质瘤中的表达水平与其恶性程度负相关，即随肿瘤的恶性程度的增高出现表达下调，TRF1可作为脑胶质瘤临床病理分级和恶性程度判断的参考指标。%Objective To explore the expression of telomeric repeat binding factor-1(TRF1) in human gliomas and normal brain tissue and its clinical significances .Methods TRF1 were detected in 58 surgical specimens of gliomas and 10 normal brain tissue by immuno-histochemical technique . Immunohistochemical scores were semi-quantified by the sum of the frequency and intensity .Results Of 58 patients with gliomas,27 were low-grade(gradeⅠ~Ⅱ) gliomas,31 were high-grade (gradeⅢ~Ⅳ) gliomas, TRF1 were expressed in all tumor samples and normal brain tissue .The level of its expression were variable .The rate of high immunohistochemical score of TRF 1 expression in low-grade malignant gliomas , high-grade malignant gliomas ,normal brain tissue were 62 .96%,29 .03%and 90 .00%respectively .The level of TRF1 expression were inversely correlated with the malignancy of gliomas.Conclusions TRF1 was expressed in both gliomas and normal brain tissues . TRF1 expression was closely related to the malignancy grade of brain tumor , it decreased in higher grade gliomas ,and may be used as a new indicator of malignancy of gliomas and meningiomas .