轻型颅脑损伤后患者血清生物标志物GFAP含量的临床研究

摘要

Objective To provide basis for diagnosis the patients with mild traumatic brain injury ( mTBI) by detecting and comparing separately the contents of the serum glial fibrillary acidic protein( GFAP ) among patients with ( mTBI ) , the patients with moderate traumatic brain injury whose glasgow coma scale ( GCS ) score is 13 -15 points and healthy control group.Methods Detected serum GFAP content by ELISA in 43 cases of patients with mTBI, 35 cases of patients with moderate traumatic brain injury whose GCS score is 13 -15 points and 20 healthy subjects, then using ROC curve analysis the results.Results There is a certain accuracy ( AUC =0.726) that serum GFAP content may distinguish between the patients with mTBI and healthy people, high accuracy( AUC=0.914) in distinguish between the patients with patients with moderate traumatic brain injury and healthy people.It has a certain accuracy (AUC=0.762) that serum GFAP content can distinguish between patients with positive and negative head CT.Conclusion The content of serum GFAP can be used as a biomarker in diagnose the patients with mTBI.It has a certain accuracy that can distinguish between patients with positive and negative head CT, and can reduce the examination of head CT in patients with mTBI.%目的 通过分别检测并比较轻型颅脑损伤(mTBI)患者、格拉斯哥昏迷量表(GCS)评分在13~15分的中型颅脑损伤患者和健康对照组血清胶质细胞纤维酸性蛋白(GFAP)含量,为临床诊断mTBI患者提供依据.方法 用ELISA法检测43例轻、35例中型颅脑损伤患者和20例健康对照组血清GFAP含量并采用ROC曲线分析,对比mTBI患者和中型颅脑损伤患者血清GFAP含量与头颅CT的关系.结果 血清GFAP含量对鉴别mTBI患者和健康人群有一定准确性(AUC=0.726);对鉴别GCS评分在13~15分的中型颅脑损伤患者和健康人群有较高准确性(AUC=0.914);血清GFAP含量对鉴别头颅CT阳性和阴性患者有一定准确性(AUC=0.762).结论 血清GFAP可作为mTBI患者诊断的生物标志物,对鉴别颅脑损伤后头颅CT阳性和阴性患者有一定准确性,可以为减少mTBI患者头颅CT检查提供依据.