HPV-DNA联合HPV-E6蛋白检测在宫颈癌筛查中的价值

摘要

目的 探讨HPV-DNA联合HPV-E6蛋白检测用于筛查宫颈癌的价值.方法 选取近期来我院门诊就诊检查的患者中选取宫颈液基细胞学检查(TCT)为阳性的患者作为观察对象,共计525例,并对以上患者分别行HPV-DNA检测、HPV-E6蛋白检测及宫颈活组织检查,比较HPV-DNA及HPV-E6蛋白两种单独检测及联合检测对于宫颈癌筛查的临床价值.结果 TCT阳性的患者中,375例为子宫颈正常或宫颈炎,134例为上皮内瘤变,14例为宫颈癌.HPV-DNA检测出的阳性率在宫颈癌前病变中均显著高于HPV-E6蛋白检测法(P＜0.05).宫颈癌的检测率均为100.0％.在上皮内瘤变和宫颈癌的检出率比较中,3组比较差异有统计学意义(P＜0.05),其中联合检测阳性率高于HPV-DNA及HPV-E6蛋白单独检测.结论 随着宫颈病变程度的加重,检测的阳性率也逐渐升高,HPV-DNA检测具有较高的灵敏度,HPV-E6蛋白检测有较高的特异度,二者联合检测可提高宫颈病变检出的灵敏度.%Objective To explore the value of HPV-DNA combined with HPV-E6 protein in the detection of cervical cancer.Methods A total of 525 patients with positive TCT were selected and treated with HPV-DNA detection,HPV detection of E6 protein and cervical biopsy.Value of HPV-DNA and HPV-E6 proteins single application and combined detection for cervical cancer screening was compared.Results In the patients with positive TCT,375 cases were normal or cervicitis,134 cases were intraepithelial neoplasia,and 14 cases were cervical cancer.The positive rate of HPV-DNA was significantly higher than that of HPV-E6 protein in cervical precancerous lesions (P ＜ 0.05).The detection rate of cervical cancer was 100％.In comparison of the detection rate of intraepithelial neoplasia and cervical cancer,there were significant differences between the three groups (P ＜ 0.05),and the positive rate of combined detection was higher than that of HPV-DNA and HPVE6 protein.Conclusion With the increasing severity of cervical lesions,the positive detection rate increases gradually.HPV-DNA has higher detection sensitivity,and detection of HPV-E6 protein has high specificity.The combined detection can improve the sensitivity of cervical lesions.