非诺贝特对胆管结扎大鼠血生化及肝组织病理学的影响

摘要

Objective To investigate the effect of Fenofibrate on serum hepatic biochemical indicators,MDA( malonaldehyde) and hepatic pathology in rats after bile duct ligation. Methods Rats were subjected to bile duct ligation to establish, the cholestasis model. Twenty - four male Wistar rats were randomly divided into two groups equally: A = bile duct ligation + normal saline ( BDL + NS) ,B = BDL + fenofibrate (30 mg/kg daily). All rats were sacrificed on 7th day after obtaining blood samples and liver tissue. Serum hepatic biochemical indicators were detected with automatic biochemistry analyzer; MD A assays were performed by spectrophotometer;Histopathological changes in liver was dyed with HE and observed under light microscope. Results There was no significant difference in terms of serum hepatic biochemical levels between group A and group B; The levels of MDA in rats of group B were significantly lower than in group A (P = 0.001 < 0. 05) ; The number of liver necrotic area in rats of group B was significantly smaller than in group A( P < 0.05 ) , The number of biliary canals in rats of group B was significantly smaller than in group A( P < 0.05). Conclusion Fenofibrate administration attenuates the levels of MOA, reduces liver necrotic area and ductular proliferation.%目的 探讨非诺贝特对胆管结扎大鼠血清肝脏生化指标、肝组织丙二醛含量及组织病理学的影响.方法 采用胆总管结扎手术制备胆汁淤积大鼠模型.24只雄性Wistar大鼠随机分为两组(每组12只):A组为胆管结扎+生理盐水组,B组为胆管结扎+非诺贝特组( 30 mg/kg).术后7d留取血标本及肝组织后处死.采用全自动生化分析仪检测肝脏生化指标；分光光度计检测肝组织丙二醛含量；光镜下观察肝脏组织病理变化.结果 两组血清肝脏生化指标无显著统计学意义；B组大鼠肝组织MDA含量显著低于A组(P＝0.001)；B组肝脏坏死面积较A组显著减轻(P＜0.05),汇管区小胆管数目较A组明显减少(P＜0.05).结论 非诺贝特可显著降低MDA含量,减少肝脏坏死面积以及小胆管增生.