不同基因型丙型肝炎病毒Core蛋白HLA-DRB1*0311、0401表位预测

摘要

Objective To predict the epitopes of hepatitis C virus(HCV) core protein of different genotypes, which can be recognized by HLA-DRB 1 *0311/0401. Methods Ten complete amino acid sequences of HCV were searched from NCBI database,which contain six genotypes of HCV. Core sequences were selected to predict secondary structure, fiexiblity, hydrophilicity, antigen index and then predict the HLA-DRB 1*0311/0401 in recognizing epitopes. Results The probable regions of epitopes according to the secondary structure, fiexiblity, hydrophilcity and antigen index of HCV core protein were 16-28, 60-74 and 98-120 respectively.For HLA-DRB1*0311, the highest scoring region in 1a/1b, 4a, 6a was 28-36, 2afo was 63-71, 3a was 29-37 and 5a was 94-102.For HLA-DRB1*0401, the highest scoring region in 1a, 2b, 4a, 6a was 84-92, 5a was 120-128, 1b was 120-128 with low score.Conclusion There were different epitopes of HCV Core protein recognized by HLA- Ⅱ between the different HCV genotypes.%目的 预测各型丙型肝炎病毒(HCV)Core区的人类白细胞抗原(HLA)-DRBI*0311及0401抗原表位.方法 从NCBI数据库中检索出十条包含六种分型的HCV完整氨基酸序列,使用生物信息学工具剪切Core区氨基酸序列并对其二级结构、可塑性、亲水性和抗原性指数进行预测,选择可能区域预测HLA-DRBI*0311及0401的抗原表位.结果 HCVCore区二级结构、可塑性、亲水性和抗原性指数预测分析可认为16～28、60～74、98～120区域或为抗原表位区域.HLA-DRB1*0311预测中,HCV-1a/b、4a、6a预测评分高的区域为28～36,HCV-2a/b为63～71,HCV-3a为29～37,HCV-5a为94～102.HLA-DRB1*0401中,HCV-1a、2b、4a、6a预测评分高区域为84～92,HCV～5a为120～128,HCV-1b中120～128可以预测到表位但评分低.结论 HCV Core区HLA-Ⅱ类分子抗原表位具有型间差异.